Acute and Sub-Acute (28-Day) Oral Toxicity Studies of Ethanolic

Aim of the study: The present study was carried out to evaluate the safety of ethanolic extract of celtis timorensis (EECT) by acute and sub-acute toxicity studies. Materials and Methods: Acute toxicity study was conducted in mice by using OECD 425 guidelines whereas sub-acute toxicity study was carried out in rats by using OECD 407 guidelines. In the acute toxicity study, mice were administered a single dose of 2000 mg/kg and 5000 mg/kg orally and then observed individually for the first four hours, then over a period of 24 hours and at least once daily for 14 days. In the subacute toxicity studies, EECT was given orally at doses of 250 mg/kg, 500 mg/kg and 1000 mg/kg body weight daily for 28 days to male and female rats respectively. General behavior, adverse effects and mortality were observed throughout the experimental period. Food intake, water intake, body weight, organ weight, hematological and biochemical parameters, histopathological changes were